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Ibisobanuro

Inzira

Inzira ya electrophoreis ni tekinoroji yo gutwikira. Ihame ryakazi ryayo nuko mubikorwa byogutanga amashanyarazi yo hanze ya DC, uduce duto twa colloidal tugenda muburyo bwerekeza kuri cathode cyangwa anode muburyo bwo gutatanya. Iyi phenomenon yitwa electrophoreis. Tekinoroji ikoresha ibintu bya electrophoreis mubintu bitandukanye byitwa electrophoreis. Ikintu cya electrophoreis cyerekana ko ibice bya colloidal bitwara umuriro w'amashanyarazi, kandi ibice bitandukanye bya colloidal bifite kamere zitandukanye na adsorb ion zitandukanye, kuburyo zitwara ibintu bitandukanye.

Inzira ya electrophoreis igabanijwemo cyane cyane anodic electrophoresis na cathodic electrophoresis. Muri anodic electrophoresis, niba ibice by'irangi byashizwemo nabi, igihangano gikoreshwa nka anode, kandi ibice by'irangi bigashyirwa kumurimo byakozwe nimbaraga zumuriro wamashanyarazi kugirango bibe byerekana firime. Ibinyuranye na byo, muri electrode ya catodiki, ibice by'irangi byashizwemo neza, igihangano gikoreshwa nka cathode, kandi ibice by'irangi na byo bigashyirwa ku kazi hifashishijwe imbaraga z'umuriro w'amashanyarazi kugira ngo habeho firime.

Inzira ya electrophoreis ifite ibyiza byinshi, nkimyenda imwe kandi nziza, kandi irashobora gupfuka hejuru y-amakoti, nkibiti bisanzwe byimbaho ​​hamwe na aluminiyumu. Byongeye kandi, gutwika amashanyarazi birashobora kuzigama amarangi nigiciro, kubera ko irangi rishobora gushyirwa neza hejuru yumurimo wakazi munsi yumuriro wamashanyarazi, bigabanya cyane imyanda yamabara. Muri icyo gihe, ibishishwa bidasanzwe hamwe n’amazi akoreshwa mu mashanyarazi ya electrophoreque birashobora gutunganywa, bikaba bitangiza ibidukikije n’ubuzima.

Nyamara, inzira ya electrophoreque nayo ifite ibibi bimwe. Ifite ibisabwa byinshi kugirango uburinganire bwuzuye, ubwiza bwubuso nuburinganire bwimikorere yibikorwa. Byongeye kandi, inzira yo gutwikira amashanyarazi iragoye cyane, kandi ibikoresho, ibipimo byo gutwikisha hamwe nibisiga irangi byamazi bigomba kubungabungwa biragoye, bisaba abashoramari babahanga kumenya.

Inzira ya electrophoreque ntabwo ikoreshwa cyane mugutwikira ibikoresho byibyuma, nkimodoka, amakamyo nibindi bicuruzwa byuma, ariko no mubinyabuzima, ubuvuzi ndetse no kwihaza mu biribwa. Mu bushakashatsi bw’ibinyabuzima n’ubuvuzi, tekinoroji ya electrophoreis ikoreshwa mu gutandukanya ibinyabuzima nka ADN, RNA na poroteyine, bifasha mu gusuzuma indwara no guteza imbere ibiyobyabwenge. Mu rwego rwo kwihaza mu biribwa, ikoranabuhanga rya electrophoreis rirashobora gukoreshwa mu kumenya ibirungo n’inyongeramusaruro mu biribwa kugira ngo ibiribwa bibe byiza.

Mugihe ukora ibikorwa bya electrophoreis, birakenewe gutegura igikoresho cya electrophoreis, ikigega cya electrophoreis na buffer ya electrophoreis, kuvanga icyitegererezo kugirango gitandukane na bffer yipakurura hanyuma ukagitera mumatara ya electrophoreis, ugashyiraho ingufu zumuriro nigihe gikwiye, ugatangira inzira ya electrophoreis, kandi usesengure ibisubizo nyuma ya electrophoreis irangiye.

Inzira ya electrophoreis ni tekinoroji yingenzi yo gutandukanya no gutandukanya ibyerekezo byinshi byo gukoresha. Hamwe nogukomeza gutera imbere mubumenyi nikoranabuhanga, inzira ya electrophoreis izarushaho kunozwa no gutezimbere, itanga uburyo bushoboka bwo gukoresha mubice bitandukanye.